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Quan-Plex™ EGFR Patient-like ctDNA Panel-In Synthetic Plasma


Description:

The Quan-Plex™ ctDNA Reference Standard is a highly-characterized, quantitative multiplex quality control that allows researchers to develop their own assays, and assess limits of detection (LODs) for ten (10) cancer-associated solid tumor EGFR mutations.  This patient-like ctDNA panel is diluted in a synthetic plasma followed by digital PCR verification of all the mutations at defined allelic frequency. It can also be used to monitor the quality of their NGS or dPCR oncology-based workflow for routine quality assessment starting from the upfront workflow steps (e.g. Isolation/extraction of circulating tumor gDNA).  This unique to market reference standards have been specifically engineered to mimic the fragmentation profile of nucleosomal ctDNA in patient samples (~166 bp).

Price: $650.00

Datasheet

Variant Information:
Gene Nucleotide Amino Acid Cosmic Frequency
EGFR 2307_2308insGCCAGCGTG V769_D770insASV COSM12376 Homozygous
EGFR 2573T>G L858R COSM6224 Homozygous
EGFR c.2369C>T p.T790M COSM6240 Homozygous
EGFR 2240_2257del18 L747_P753>S COSM12370 Homozygous
EGFR c.2281G>T p.D761Y COSM21984 Homozygous
EGFR 2582T>A L861Q COSM6213 Homozygous
EGFR 2236_2250del15 E746_A750delELREA COSM6225 Homozygous
EGFR 2303G>T S768I COSM6241 Homozygous
EGFR 2155G>A G719S COSM6252 Homozygous
EGFR c.2389T>A p.C797S COSM6493937 Homozygous
General Information:
CatalogARF-1006CTP
Size1 panel
FormatctDNA
Volume/Dimension4 vials of 500µl each
Quantity300 ng/vial
Concentration0.6 ng/µl
Cell(s)RKO
Shelf Life2 years
BufferTris-EDTA (10 mM Tris-HCl, 1 mM EDTA), pH 8.0
Shipping TemperatureGel packs
Storage Temperature-20°C (2 years/Exp Date)
Quality ControlGenotype: Sanger sequencing of locus-specific PCR (cell line)
Quality: Bio-Analyzer™ 1000 Chip and NGS (Ion AmpliSeq Hotspot Cancer Panel V2 on Illumina) DNA Quantification: Qubit 4.0 fluorometer
Mutation Allelic Frequency: Digital PCR **Quantified with NGS. Note multiple copies of the insertion may be observed with NGS. Manufacturing: ISO 9001:2015 and ISO 13485:2016 Certified
Freeze/ThawNo more than 3 cycles
RegulatoryResearch Use Only. Not Intended for human or animal diagnostic or therapeutic use.
RestrictionsThis is a limited use product. This product, any material that contains this product in whole or in part, any progeny, modification or derivative of this product, any cell or animal made or modified by using this product, and any progeny, modification or derivative of such cell or animal may not be transferred by the purchaser to any other person, entity or any of the above to perform services for the benefit of any other person or entity or for commercial purpose of any kind. This product may only be used by the purchaser for its internal research for use as a research tool for research purposes.
Catalog: ARF-1006CTP
Price: $650.00
Availability: Comming Soon
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