Info@accuref.com
1-866-497-4180 (U.S Toll Free)
1-408-773-8007
Accuref logo s

Quan-Plex™ EGFR C7979S/T790M (Cis) FFPE Cell Scroll 5%


Description:

The Quan-Plex™ EGFR C7979S/T790M (cis) FFPE Cell Scroll (5%) is a highly-characterized, quantitative multiplex FFPE quality control enabling researchers to optimize their nucleic acid extraction protocol for optimal sensitivity or to assess extraction reagent lot variability prior to downstream genetic analysis (e.g. next generation sequencing (NGS) or dPCR genetic analysis).  The two EGFR co-mutations (Cis) has been implicated in Thyrosine Kinase Inhibitor (TKI) therapeutics and drug resistance.


Datasheet

Certificate of Analysis
ARF1004S281037-5

Variant Information:
Gene Nucleotide Amino Acid Cosmic Frequency
EGFR c.2369C>T p.T790M COSM6240 5%
EGFR c.2389T>A p.C797S COSM6493937 5%
General Information:
Catalog ARF-1004S-5
Size 1 scroll
Format FFPE
Volume/Dimension 20 µm section with a 7.0 mm diameter cell pellet core
Quantity 1
Concentration >/= 60% cell density; >/= 400ng/scroll using Maxwell LEV Plus FFPE DNA Extraction Kit
Cell(s) RKO
Shelf Life 24 months
Buffer Tris-EDTA (10 mM Tris-HCl, 1 mM EDTA), pH 8.6
Shipping Temperature Ambient
Fixation Process FFPE Fixation:10% BFS, 24hrs at room temperature
Storage Temperature -20°C (2 years Exp Date)
Quality Control Genotype: Sanger sequencing of locus-specific PCR (cell line)
Quality: H&E staining of 5µm slide
Mutation Allelic Frequency: Digital PCR. Tested with NGS (Ion AmpliSeq Hotspot Cancer Panel V2 on Illumina) but not used for QC release. Manufacturing: ISO 9001:2015 and ISO 13485:2016 Certified
Freeze/Thaw Extracted DNA: No more than 3 cycles
Regulatory Research Use Only. Not Intended for human or animal diagnostic or therapeutic use.
Restrictions This is a limited use product. This product, any material that contains this product in whole or in part, any progeny, modification or derivative of this product, any cell or animal made or modified by using this product, and any progeny, modification or derivative of such cell or animal may not be transferred by the purchaser to any other person, entity or any of the above to perform services for the benefit of any other person or entity or for commercial purpose of any kind. This product may only be used by the purchaser for its internal research for use as a research tool for research purposes.
Arf 1004s 5
(Click Image To Enlarge)

Catalog: ARF-1004S-5
© 2019 Copyright AccuRef Diagnostics, a Division of Applied StemCell Inc.