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Quan-Plex™ NGS FFPE Cell Scroll Reference Standard


Description:

The Quan-Plex™ NGS FFPE Cell Scroll Reference Standard is a highly-characterized, quantitative multiplex, reference standard that allows researchers to accurately assess the quality of an oncology-based next-generation sequencing (NGS), digital PCR, and/or real-time PCR workflow using CRISPR/cas9 genome-edited cancer cell lines prepared into a formalin-fixed paraffin-embedded (FFPE) mimetic cell block which was then sectioned into 20μm scrolls. This reference standard panel spans a broad range (1% to 10%) of actionable mutations at digital PCR and NGS-verifed mutation allelic frequencies (%MAF). In addition, the Quan-Plex™ NGS FFPE Cell Scroll Reference Standard contains > 1,500 variants that are endogenous to the isogenic parental cancer cell lines (RKO and HCT116). This convenient, easy-to-use panel includes nine (9) actionable somatic mutations across six oncogenes (KIT, EGFR, NRAS, HRAS, KRAS and PIK3CA).

Price: $375.00

Datasheet

Certificate of Analysis
F1001S291814

Variant Information:
Gene Nucleotide Amino Acid Cosmic Frequency
EGFR 2573T>G L858R COSM6224 Homozygous
EGFR c.2369C>T p.T790M COSM6240 Homozygous
HRAS c.182A>G p.Q61R COSM499 Homozygous
KIT c.2447A>T p.D816V COSM1314 Homozygous
KRAS 35G>A G12D COSM521 Homozygous
NRAS c.181C>A p.Q61K COSM580 Homozygous
PIK3CA c.1633G>A p.E545K COSM763 Homozygous
PIK3CA c.3140A>T p.H1047L COSM776 Homozygous
EGFR 2155G>A G719S COSM6252 Homozygous
General Information:
CatalogARF-1001S
Size20 µm cell scroll
FormatFFPE
Volume/Dimension20 µm section with a 7.0 mm diameter cell pellet core
Quantity1
Concentration>/= 60% cell density; >/= 400ng/scroll using Maxwell LEV Plus FFPE DNA Extraction Kit
Cell(s)RKO, HCT116
Shelf Life36 months
BufferTris-EDTA (10 mM Tris-HCl, 1 mM EDTA), pH 8.0
Shipping TemperatureAmbient
Fixation Process10% buffered formalin solution (4% formaldehyde) for 24 hours at room temperature
Storage Temperature-20°C
Quality ControlGenotype: Sanger sequencing of locus-specific PCR (cell line)
Quality: >/= 60% cell density assessed by digital image analysis of H&E staining of 5µm section, >/= 400ng/scroll using Promega Maxwell LEV Plus FFPE DNA Extraction Kit
DNA Quantification: Qubit 4.0 fluorometer
Mutation Allelic Frequency: Digital PCR and NGS (Ion AmpliSeq Hotspot Cancer Panel V2 on Illumina) Manufacturing: ISO 9001:2015 and ISO 13485:2016 Certified
Freeze/ThawExtracted FFPE gDNA: No more than 3 cycles
RegulatoryResearch Use Only. Not Intended for human or animal diagnostic or therapeutic use.
RestrictionsThis is a limited use product. This product, any material that contains this product in whole or in part, any progeny, modification or derivative of this product, any cell or animal made or modified by using this product, and any progeny, modification or derivative of such cell or animal may not be transferred by the purchaser to any other person, entity or any of the above to perform services for the benefit of any other person or entity or for commercial purpose of any kind. This product may only be used by the purchaser for its internal research for use as a research tool for research purposes.
Catalog: ARF-1001S
Price: $375.00
Availability: Comming Soon
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