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Quan-Plex™ NGS Reference Standard Genomic DNA


Description:

The Quan-Plex™ NGS Reference Standard is a highly-characterized, quantitative multiplex NGS control that allows researchers to accurately assess their oncology-based next-generation sequencing (NGS) workflow. The reference standard panel spans a broad range of actionable mutations at dPCR-validated allelic frequencies ranging from 1% to 30%.  In addition, the Quan-Plex™ NGS Reference Standards contains > 1,500 COSMIC variants that are endogenous to the isogenic parental cell lines. Digital PCR-validated variants include 11 actionable somatic mutations across seven oncogenes (BRAF, KIT, EGFR, NRAS, HRAS, KRAS and PIK3CA).

Price: $200.00

Datasheet

Certificate of Analysis
ARF1001G291539-1 ARF1001G291539-1

Variant Information:
Gene Nucleotide Amino Acid Cosmic Frequency
BRAF 1799T>A V600E COSM476 30%
EGFR 2573T>G L858R COSM6224 10%
EGFR c.2369C>T p.T790M COSM6240 1%
HRAS c.182A>G p.Q61R COSM499 3%
KIT c.2447A>T p.D816V COSM1314 10%
KRAS 35G>A G12D COSM521 2%
NRAS c.181C>A p.Q61K COSM580 5%
PIK3CA c.1633G>A p.E545K COSM763 7%
PIK3CA c.3140A>T p.H1047L COSM776 6%
EGFR 2235_2249del15 E746_A750delELREA COSM6223 5%
EGFR 2155G>A G719S COSM6252 5%
General Information:
CatalogARF-1001G-1
Size1 vial
FormatgDNA
Volume/Dimension20 µl
Quantity1 µg
Concentration50 ng/µl
Cell(s)HCT116, RKO
Shelf Life24 months
BufferTris-EDTA (10 mM Tris-HCl, 1 mM EDTA), pH 8.0
Shipping TemperatureGel packs
Storage Temperature-20°C (1 years Exp Date)
Quality ControlGenotype: Sanger sequencing of locus-specific PCR (cell line)
Quality: Bio-Analyzer™ 1000 Chip
DNA Quantification: Qubit 4.0 fluorometer
Mutation Allelic Frequency: Digital PCR and NGS (Ion AmpliSeq Hotspot Cancer Panel V2 on Illumina) Manufacturing: ISO 9001:2015 and ISO 13485:2016 Certified
Freeze/ThawNo more than 3 cycles
RegulatoryResearch Use Only. Not Intended for human or animal diagnostic or therapeutic use.
RestrictionsThis is a limited use product. This product, any material that contains this product in whole or in part, any progeny, modification or derivative of this product, any cell or animal made or modified by using this product, and any progeny, modification or derivative of such cell or animal may not be transferred by the purchaser to any other person, entity or any of the above to perform services for the benefit of any other person or entity or for commercial purpose of any kind. This product may only be used by the purchaser for its internal research for use as a research tool for research purposes.
Catalog: ARF-1001G-1
Price: $200.00
Availability: In Stock
© 2018 Copyright AccuRef Diagnostics, a Division of Applied StemCell Inc.
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